Use of TaqMan Real-Time Reverse Transcription-PCR for Rapid Detection, Quantification, and Typing of Norovirus
نویسندگان
چکیده
منابع مشابه
Use of TaqMan real-time reverse transcription-PCR for rapid detection, quantification, and typing of norovirus.
Noroviruses (NoVs) are the most commonly identified cause of outbreaks and sporadic cases of acute gastroenteritis. We evaluated and optimized NoV-specific TaqMan real-time reverse transcription (RT)-PCR assays for the rapid detection and typing of NoV strains belonging to genogroups GI and GII and adapted them to the LightCycler platform. We expanded the detection ability of the assays by deve...
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The SD of residuals (S y͉x) was 73 ng/L in the concentration range of 0 –5000 ng/L and 22 ng/L in the range of 0 –500 ng/L. The agreement between the GC/MS and MEIA assays indicated low interference from other estrogenic compounds and their metabolites in the latter assay. In conclusion, we have developed a GC/MS method for the quantification of estradiol in patient serum samples and serum-based...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2006
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.44.4.1405-1412.2006